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Monday, March 22, 2010

wHat A mEss!!!!!!!!

Really dunno what a "beep" things happens to me...
seems like there's been a curse here and there till everything i do got to be wrong!!!!!!!!

Starting when i'm doing my work in the morning till evening...
wrong sample in wrong tube, but then i successfully cover with....
then another thing happen!!!!The Biggest One!!!!!
D***, dunno where the "beep" word came from..but it came out from my mouth!!!!
wrong volume!!!!u really in big mess with the DNA!!!!!!!

Luckily, then my supervisor said : " it's ok, maybe we can try an error"
a bit relief..but still nerves!!! thinking what will happen to my band when undergo electrophoresis.
then, i put all samples in the mastercycler....

happily continue my work after lunch, preparing agar.....
then, come the hard part which is dealing with the EtBr (chemical that can brake down your DNA processes)....
happily, done another mistake while pouring the agar containing that EtBr!!!!
I pray to god " ya allah, mintak-mintak la kawen nanti still dapat anak"
hehehehhee...........

then, i hope i'm not doing other mistakes........but...................
i dunno what am i thinking!!!!!!!!!!!
so, "beep".........mamai, pikiran melayang!!!!!!! seems im not there!!!!
again, i'm forget bout the -ve and +ve control in the agar!!!!

Then. again kak iza said " i told u, samples go first, then -ve control, marker then +ve control"....
feels ashamed...again!!!!
hope still got good result for virus lab!!!!

After so much tired....and a bit overtime, my band seems so well done!!!!!!!!!!
no unspecified band, no contamination and nothing!!!!
GOOD JOB GIRL!!!!!!!

hahahaa love PCR & electrophoresis soooooooo much....................But LOVE HIM EVEN MORE!!!!!!!!

wish could see u again!!!!!!( so taman!!!!!gedixx,xde kne mengene ngan post!!!!!)

till then,
bubbye

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